Plasmid

Part:BBa_K4959004:Design

Designed by: Liang Chenyu   Group: iGEM23_ULC   (2023-10-06)


SgaMcrA-pET28a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4554
    Illegal EcoRI site found at 4971
    Illegal PstI site found at 5523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4554
    Illegal EcoRI site found at 4971
    Illegal PstI site found at 5523
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4554
    Illegal EcoRI site found at 4971
    Illegal BglII site found at 4402
    Illegal BglII site found at 5578
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4554
    Illegal EcoRI site found at 4971
    Illegal PstI site found at 5523
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4554
    Illegal EcoRI site found at 4971
    Illegal PstI site found at 5523
    Illegal NgoMIV site found at 2622
    Illegal NgoMIV site found at 2782
    Illegal NgoMIV site found at 4370
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

E. coli

References